...2 of the human interleukin‐1 receptor antagonist (IL‐1Ra...

来自 : 发布时间：2024-05-17

Single nucleotide polymorphisms in Intron 2 of the human interleukin‐1 receptor antagonist (IL‐1Ra) gene: further definition of the IL‐1β and IL‐1Ra polymorphisms in North American Caucasians and Taiwanese Chinese - Tseng - 2001 - Tissue Antigens - Wiley Online Library Single nucleotide polymorphisms in Intron 2 of the human interleukin-1 receptor antagonist (IL-1Ra) gene: further definition of the IL-1β and IL-1Ra polymorphisms in North American Caucasians and Taiwanese Chinese L.-H. Tseng, Department of Medical Genetics, Pathology and Oncology, National Taiwan University Hospital, and Graduate Institutes of Clinical Medicine and Cancer Research Center, National Taiwan University, Taipei, TaiwanSearch for more papers by this authorP.-J. Chen, Department of Medical Genetics, Pathology and Oncology, National Taiwan University Hospital, and Graduate Institutes of Clinical Medicine and Cancer Research Center, National Taiwan University, Taipei, TaiwanSearch for more papers by this authorM.-T. Lin, Department of Medical Genetics, Pathology and Oncology, National Taiwan University Hospital, and Graduate Institutes of Clinical Medicine and Cancer Research Center, National Taiwan University, Taipei, Taiwan Human Immunogenetics Program, Division of Clinical Research, Fred Hutchinson Cancer Research Center, Seattle, Washington, USASearch for more papers by this authorW.-Y. Shau, Department of Medical Genetics, Pathology and Oncology, National Taiwan University Hospital, and Graduate Institutes of Clinical Medicine and Cancer Research Center, National Taiwan University, Taipei, TaiwanSearch for more papers by this authorS.-M. Chaung, Department of Medical Genetics, Pathology and Oncology, National Taiwan University Hospital, and Graduate Institutes of Clinical Medicine and Cancer Research Center, National Taiwan University, Taipei, TaiwanSearch for more papers by this authorP.J. Martin, Human Immunogenetics Program, Division of Clinical Research, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA Department of Medicine, University of Washington, Seattle, Washington, USASearch for more papers by this authorJ.A. Hansen, Human Immunogenetics Program, Division of Clinical Research, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA Department of Medicine, University of Washington, Seattle, Washington, USASearch for more papers by this author L.-H. Tseng, Department of Medical Genetics, Pathology and Oncology, National Taiwan University Hospital, and Graduate Institutes of Clinical Medicine and Cancer Research Center, National Taiwan University, Taipei, TaiwanSearch for more papers by this authorP.-J. Chen, Department of Medical Genetics, Pathology and Oncology, National Taiwan University Hospital, and Graduate Institutes of Clinical Medicine and Cancer Research Center, National Taiwan University, Taipei, TaiwanSearch for more papers by this authorM.-T. Lin, Department of Medical Genetics, Pathology and Oncology, National Taiwan University Hospital, and Graduate Institutes of Clinical Medicine and Cancer Research Center, National Taiwan University, Taipei, Taiwan Human Immunogenetics Program, Division of Clinical Research, Fred Hutchinson Cancer Research Center, Seattle, Washington, USASearch for more papers by this authorW.-Y. Shau, Department of Medical Genetics, Pathology and Oncology, National Taiwan University Hospital, and Graduate Institutes of Clinical Medicine and Cancer Research Center, National Taiwan University, Taipei, TaiwanSearch for more papers by this authorS.-M. Chaung, Department of Medical Genetics, Pathology and Oncology, National Taiwan University Hospital, and Graduate Institutes of Clinical Medicine and Cancer Research Center, National Taiwan University, Taipei, TaiwanSearch for more papers by this authorP.J. Martin, Human Immunogenetics Program, Division of Clinical Research, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA Department of Medicine, University of Washington, Seattle, Washington, USASearch for more papers by this authorJ.A. Hansen, Human Immunogenetics Program, Division of Clinical Research, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA Department of Medicine, University of Washington, Seattle, Washington, USASearch for more papers by this author Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URLShare a linkShare onEmailFacebookTwitterLinked InRedditWechat Abstract: Previous studies have suggested that a variable number tandem repeat (VNTR) polymorphism in the second intron of the interleukin-1 receptor antagonist (IL-1Ra) gene and the single nucleotide polymorphisms at positions −511 and +3954 of the IL-1β gene might be associated with increased risks of chronic inflammatory diseases, autoimmune diseases and gastric cancer. In the present study, IL-1β and IL-1Ra genotypes were analyzed among Asians in Taiwan and Caucasians in North America. We identified a novel polymorphism with 3 nucleotide substitutions in the IL-1Ra VNTR 2-repeat allele. One of the substitutions corresponds with the fourth 3′ end nucleotide of the reverse primer that is often used for analysis of the IL-1Ra-associated VNTR locus. Mismatching between this primer and the 2-repeat allele can cause misleading amplification results when stringent conditions are used for annealing. The estimated haplotype frequencies of the variant IL-1 genes were significantly different between Taiwanese and Caucasians. The frequency of the pro-inflammatory IL-1Ra 2-repeat allele was significantly lower in Taiwanese than in Caucasians. In contrast, the frequencies of the pro-inflammatory IL-1β–511T allele and +3954C allele were significantly higher among Taiwanese compared with Caucasians. The full text of this article hosted at iucr.org is unavailable due to technical difficulties. Please check your email for instructions on resetting your password. If you do not receive an email within 10 minutes, your email address may not be registered, and you may need to create a new Wiley Online Library account. Can\'t sign in? Forgot your username? Enter your email address below and we will send you your username If the address matches an existing account you will receive an email with instructions to retrieve your username